Feng Wang/王峰 Ph.D.

·        Address: No.256, Tiansheng Road, Beibei, Chongqing, China, 400715

·        Email: atp_bomb@163.com，wangf1986@swu.edu.cn

·        Tel: (+86) 23-68251987

·        Position: Research associate in College of biotechnology, Southwest University, Chongqing, People’s Republic of China(PRC)

EDUCATION AND WORK EXPERIENCE

·       Research associate (2016-): College of biotechnology, Southwest University, Chongqing, China.

·        Postdoctor (2014-2016): College of biotechnology, Southwest University, Chongqing, China.

·        Ph.D. (2008-2014): Biochemistry and Molecular Biology, State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing, China.

·      Bachelor Degree. (2004-2008): Bioengineering, College of life science, Southwest university, Chongqing, China.

RESEARCH FIELDS

·        Gene expression regulation and genome modification of silkworm or insect species using transposase, site-specific integrases, customized ZFN, TALEN and CRISPER/Cas9 nucleases

·        Genetically engineering the silkworm spun silk as functional biomaterials.

·        Engineering the silk gland of silkworm Bombyx.mori as bioreactor to produce recombinant pharmaceutical proteins.

PUBLICATIONS

1. Feng Wang, Riyuan Wang, Yuancheng Wang, Ping Zhao, Qingyou Xia^*. Large-scale production of bioactive recombinant human acidic fibroblast growth factor in transgenic silkworm cocoons. Scientific Reports. 5:16323(2015). http://www.nature.com/articles/srep16323

2. Feng Wang, Hanfu Xu, Yuancheng Wang, Riyuan Wang, Lin Yuan, Huan Ding, Chunnuan Song, Zhixin Peng, Zhangchuan Peng, Sanyuan Ma, Ping Zhao, Qingyou Xia*. Advanced silk material spun by a transgenic silkworm promotes cell proliferation for biomedical application. Acta Biomat. December 2014, 10(12): 4947-4955. http://www.sciencedirect.com/science/article/pii/S1742706114002876

3. Feng Wang, Hanfu Xu, Lin Yuan, Sanyuan Ma, Yuancheng Wang, Xiaoli Duan, Jianping Duan, Zhonghuai Xiang, Qingyou Xia*. An optimized sericin-1 expression system for mass-producing recombinant proteins in the middle silk glands of transgenic silkworms. Transgenic Res. 2013 Oct; 22(5): 925-38.  Link: http://link.springer.com/article/10.1007/s11248-013-9695-6

4. Feng Wang, Sanyuan Ma, Hanfu Xu, Jianping Duan, Yuancheng Wang, Huan Ding, Yuanyuan Liu, Xiaogang Wang, Ping Zhao, Qingyou Xia*. High-efficiency system for construction and evaluation of customized TALENs for silkworm genome editing. Mol Genet Genomics. 2013 Dec; 288(12):683-90. Link: http://link.springer.com/article/10.1007/s00438-013-0782-4

5. Feng Wang,Riyuan Wang, Yuancheng Wang, Hanfu Xu, Sanyuan Ma, Lin Yuan, HuanDing, Ping Zhao, Qingyou Xia*. Remobilizing deleted piggyBac vector post integration for transgene stability in silkworm. Mol Genet Genomics. 2015 Jun; 290 (3):1181-1189. http://link.springer.com/article/10.1007/s00438-014-0982-6

6. Yuancheng Wang, Feng Wang, Riyuan Wang, Ping Zhao, Qingyou Xia* . 2A self-cleaving peptide-based multi-gene expression system in the silkworm Bombyxmori. Scientific Reports. 5:16273(2015). http://www.nature.com/articles/srep16273

7. Hanfu Xu, Lin Yuan, Feng Wang, Yuancheng Wang, Riyuan Wang, Chunnuan Song, Qingyou Xia, Ping Zhao. Overexpression of recombinant infectious bursal disease virus (IBDV) capsid protein VP2 in the middle silk gland of transgenic silkworm. Transgenic Res. 2014, 23(5): 809-816.http://link.springer.com/article/10.1007/s11248-014-9827-7

8. Duan J, Meng X, Ma S, Wang F, Guo H, Zhang L, Zhao P, Kan Y, Yao L, Xia Q. The C-terminus of DSXF5 protein acts as a novel regulatory domain in Bombyxmori. Transgenic Res. 2016 Mar 15.http://link.springer.com/article/10.1007/s11248-016-9946-4

9. Sanyuan Ma, Shengling Zhang, Feng Wang, Yong Liu, Yuanyuan Liu, Hanfu Xu, Chun Liu, Ying Lin, Ping Zhao, Qingyou Xia*. Highly efficient and precise genome editing in silkworm using custom TALENs. PLoS One September 18, 2012. 7(9): e45035. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0045035

10. Hanfu Xu, Yaowen Liu, Feng Wang, Lin Yuan, Yuancheng Wang, Sanyuan Ma, Helen Beneš, QingYou Xia*. Overexpression and functional characterization of an Aspergillus nigerphytase in the fat body of transgenic silkworm, Bombyxmori. Transgenic Res. 2014, 23(4): 669-677.http://link.springer.com/article/10.1007/s11248-014-9797-9

11. DangJun Deng, HanFu Xu, Feng Wang, Xiaoli Duan, SanYuan Ma, ZhongHuai Xiang &QingYou Xia. The promoter of Bmlp3 gene can direct fat body-specific expression in the transgenic silkworm, Bombyxmori. Transgenic Res. 2013 Oct; 22(5): 1055-63.http://link.springer.com/article/10.1007/s11248-013-9705-8

12. Jianping Duan, Hanfu Xu, Feng Wang, Sanyuan Ma, XingfuZha, Huizhen Guo, Ping Zhao, Qingyou Xia*. Novel female-specific trans-spliced and alternative splice forms of dsx in the silkworm Bombyxmori. Biochemical and Biophysical Research Communications. 431 (2013) 630–635.http://www.sciencedirect.com/science/article/pii/S0006291X12024102

13. Jianping Duan, Hanfu Xu, Shangyuan Ma, Huizheng Guo, Feng Wang, Liying Zhang, XingfuZha, Ping Zhao and Qingyou Xia*. Ectopic expression of the male BmDSX affects formation of the chitin plate in female Bombyxmori. MolReprod Dev. 2014 Mar; 81(3): 240-7.http://onlinelibrary.wiley.com/doi/10.1002/mrd.22290/full

14. Jianping Duan, Hanfu Xu, Huizhen Guo, David A. O'Brochta, Feng Wang, Sanyuan Ma, Liying Zhang, XingfuZha, Ping Zhao, Qingyou Xia*. New insights into the genomic organization and splicing of the doublesex gene, a terminal regulator of sexual differentiation in the silkworm Bombyxmori. PLoS One. 2013 Nov 7; 8(11): e79703. Doi: 10.1371/journal.pone.0079703.http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0079703

15. Sanyuan Ma, Xiaogang Wang, Jitao Fei, Yuanyuan Liu, Jianping Duan, Feng Wang,Hanfu Xu, Ping Zhao, Qingyou Xia*. Genetic marking of sex using a W chromosome-linked transgene. Insect BiochemMol Biol. 2013 Dec; 43(12): 1079-86.http://www.sciencedirect.com/science/article/pii/S0965174813001562

16. Jianping Duan, Hanfu Xu, Sanyuan Ma, Huizhen Guo, Feng Wang, Ping Zhao, Qingyou Xia*. Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms. Transgenic Res. 2013 Jun; 22(3): 607-19.http://link.springer.com/article/10.1007/s11248-012-9677-0

17. Hanfu Xu, Dangjun Deng, Lin Yuan, Yuancheng Wang, Feng Wang, Qingyou Xia*. Identification of a functional element in the promoter of the silkworm (Bombyxmori) fat body-specific gene Bmlp3. Gene, 2014, 546(1): 129-134.http://www.sciencedirect.com/science/article/pii/S0378111914006015

18.  王峰，马三垣，赵萍，夏庆友. 转基因家蚕丝腺生物反应器体系的建立和应用 蚕业科学 2014, 40(5): 0757-0763.

19.  段建平，徐汉福，马三垣，邓党军，王峰，夏庆友. 人脑源性神经营养因子基因(hBDNF)在转基因家蚕丝腺中的特异表达 蚕业科学 2009 35(2).

20.  徐汉福，王峰，王根洪，段晓利，马三垣，夏庆友. 家蚕Poly(A)结合蛋白基因BmPABP的克隆及序列与功能分析 蚕业科学 2011 37(3).

21.  邓党军，徐汉福，王峰，马三垣，夏庆友. BmLSP基因启动子驱动DsRed在转基因家蚕中的表达分析 蚕业科学 2011 37(2).

22.  徐汉福，马三垣，王峰，刘耀文，夏庆友. 适用于实用家蚕品种转基因的蚕卵滞育解除方法 蚕业科学 2011 37(1).

23.  徐汉福，幸俊逸，王峰，夏庆友. 外源piggyBac转座元件在转基因家蚕中的整合位点分析 蚕学通讯 2010 30(1).

CHINESE PATENT:

1. 增强子hr3. 夏庆友，王峰，徐汉福，马三垣，赵萍，向仲怀.（已授权）ZL201110423280.3.

2. 转录激活因子IE1. 夏庆友，王峰，徐汉福，马三垣，赵萍，向仲怀.（已授权）ZL201110423279.0.

3. 家蚕PABP结合蛋白互作因子基因 BmPaip1及其重组表达载体和应用. 夏庆友，王峰，徐汉福，袁林，王元成，赵萍，向仲怀.（已授权）ZL201310030799.4.

4. 编辑家蚕基因组TALE重复区的高效组装方法及其骨架载体. 夏庆友，王峰，徐汉福，马三垣，王元成，袁林，赵萍，向仲怀. （已授权）ZL201310049848.9.

5. 家蚕表皮蛋白BmCP231启动子及其重组表达载体和应用.夏庆友，衣启营，赵萍，王鑫，马三垣，王峰.（已授权）ZL201210085897.3.

6. 改造的人酸性成纤维细胞生长因子基因及其重组载体和应用, 申请日2013-10-22，发明人：夏庆友；王峰；徐汉福；王元成；袁林；马三垣；赵萍，ZL201310450300.5 (已授权)；

7. 实现家蚕转基因长期稳定性的piggyBac载体及其制备方法. 夏庆友，王峰，徐汉福，王元成，王日远，赵萍. （已授权）zl201410177428.3；

8. 适于家蚕丝腺表达的改造人酸性成纤维细胞生长因子基因及其表达系统和应用.夏庆友，王峰，徐汉福，王日远，赵萍.（已授权）zl201410177732.8；

9. 适于家蚕中部丝腺表达的鸡传染性法氏囊病病毒结构蛋白VP2及其表达载体和应用,申请日2014-6-20，发明人：赵萍，袁林，王峰，徐汉福，夏庆友,申请号：（已授权）201410280164.4。

10. 一种适用于含绿色荧光蛋白茧丝的脱胶方法。发明人：席星航,杨佩,王峰,赵萍,夏庆友。申请号：（已授权）ZL201410805499.3。

FUNDS：

1.  主持国家自然科学基金_青年科学基金项目：高效家蚕丝腺生物反应器与蚕丝遗传改良创新研究；批准时间：2015年8月18日。2016/01-2018/12。

2.  主持中国博士后科学基金第八批特别资助：新型蚕丝遗传改良与素材创新研究；批准时间：2015年7月6日，2015/07-2016/07。

3.  主持重庆市博士后科研项目特别资助,家蚕丝腺生物反应器与蚕丝遗传改良应用研究。2015/12-2016/07。

4.  主持2016年中央高校基本业务费_一般项目：人工核酸酶介导的定点表达技术在家蚕丝腺生物反应器中的建立和应用；批准时间：2015年9月17日。2016/01-2017/12。

5.  主持主持重庆市蚕丝纤维新材料工程技术中心开放课题：家蚕丝腺重组表达细胞生长因子及其在蚕丝面膜中的应用研究。2016/06-2017/06。

6.  主持西南大学研究生科技创新基金_优博项目。

ACADEMIC REVIEWER:

Journal of Materials Chemistry B,

Journal of Agricultural Science and Technology (JAST),

World Journal of Agricultural Research.